FIG 4.

Biomass accumulation, glucose concentration in the feed, and viability of two independent glucose-limited aerobic retentostat cultures of P. pastoris. Retentostat cultures were initiated from chemostat cultures at time zero. Shown are the predicted biomass accumulation profile (short dashed line), measured dry biomass concentration (open circles), biomass concentration corrected for viability (closed circles), predicted glucose concentration (solid line), measured glucose concentration in the mixing vessel (closed triangles), and culture viability based on fluorescence staining (open diamonds).