TABLE 1.
Primers used in this study
| Primera | Sequence (5′ to 3′) | Efficiency |
|---|---|---|
| katF | CGCACGGGAAATTTGTTACT | 2.05 |
| katR | GGTCAGGCTTCAAGGAATGA | |
| lmo0367F | TCATGTGCGCCTAGCCAAAG | 2.10 |
| lmo0367R | CCAATTGGGACGGTGTATTC | |
| lmo1604F | AGGCACACAAGCTCCAAGAT | 1.93 |
| lmo1604R | CGCAGCAAGAGGGTAGTTTA | |
| tpxF | GCGTTGTTCCTTCCATTGAT | 1.96 |
| tpxR | ATCGCGGTGGTCAGATAAAG | |
| 16SF | TGGGGAGCAAACAGGATTAG | 2.27 |
| 16SR | TAAGGTTCTTCGCGTTGCTT |
a
Primers were designed based on the L. monocytogenes EGD-e published sequences from the ListiList website (http://genolist.pasteur.fr/ListiList/) except katF, katR, 16SF, and 16SR, which were designed based on the Listeria monocytogenes 10403S cont5.3 whole-genome shotgun sequence (http://www.ncbi.nlm.nih.gov/genome/159?genome_assembly_id=159669). All primers designed on EGD-e background were confirmed to contain no mismatches with the 10403S background using Standard Nucleotide Blast.