TABLE 2.

Cell wall compositions of wild-type, msn2Δ msn4Δ, and yap1Δ yeast strains upon exposure to 9% (vol/vol) ethanol for 5 h^a

Polysaccharide	% of total cell wall polysaccharides (mean ± SD)d
	BY4741 (wild type)		BY msn2Δ msn4Δ		BY yap1Δ		BYMSN2up
	Control	With ethanol	Control	With ethanol	Control	With ethanol	Control	With ethanol
β-(1,3)-Glucan	36.8 ± 5.0	37.7 ± 2.0	42 ± 6.0	38.5 ± 2.5	ND	ND	37.2 ± 5.5	36.7 ± 5.0
β-(1,6)-Glucan	20 ± 1.0	21 ± 2.0	23 ± 2.0	24 ± 2.0	ND	ND	22.8 ± 4.0	26.3 ± 5.0
β-Glucanb	57 ± 1.0	59 ± 4.0	65 ± 5.0#	63.5 ± 4.5#	58 ± 6.0c#	60 ± 5.0	57.4. ± 5.5	63.0 ± 5.0
Chitin	4.9 ± 0.2	4.9 ± 0.2	4.2 ± 0.2	3.5 ± 0.2	3.0 ± 0.5	3.2 ± 0.3	3.8 ± 0.3	3.5 ± 0.32
Mannan	37.4 ± 3.3	36 ± 3.1	32 ± 1.0#	33 ± 2.0#	39 ± 3.0#	36 ± 2.0	38.9 ± 4.0	36.5 ± 5.0
Glucan/mannan ratio	1.52	1.63	2.1	1.95	1.49	1.67	1.52	1.72

^aCell wall compositions were determined for exponentially growing yeast cells in YPD, treated or not for 5 h with 9% ethanol as described in Materials and Methods. Values are means and SD for three independent biological samples that were technically repeated two times.

^bSum of β-(1,3)- and β-(1,6)-glucan.

^cDetermined by a chemical method.

^d#, P < 0.05 (statistical difference between wild-type and mutant yeast cells as calculated by Student's t test); ND, not determined.