FIG 1.

Motility of E. coli strains on 0.25% (wt/vol) LB agar at 37°C for 16 h (A) and at 15°C for 40 h (B), and biofilm formation in LB broth and SMM (C). All soil-persistent and commensal strains were significantly (P < 0.0001) more motile than the laboratory strain BW25113 at 15°C and 37°C. The wild-type BW25113 strain was significantly (P < 0.0003) less motile than the BW25113ΔrpoS strain at 37°C but not at 15°C. Biofilm was produced in rich and minimal media, with more biofilm produced at 15°C than at 37°C. UTI, urinary tract infection-associated E. coli isolate. Error bars represent standard deviations from three independent replicates. Data with similar lowercase or uppercase letters are not significantly different (P > 0.05).