FIG 4.

Peptide inhibitor has a suppressive effect on conjugation frequency. Overnight cultures of donors and recipients were diluted 1:10 and mixed to achieve two sets with a D/R ratio of 0.01:1 (low donor-to-recipient ratio). One set of mating mixtures served as a control. The other set was treated with inhibitor peptide. The conjugation frequency is represented by the number of transconjugants per donor. (A) Liquid mating of pAD1 with and without 50 ng/ml of iAD1; (B) liquid mating of pAM373 with and without 500 ng/ml of iAM373. Data shown in panels A and B are averages of results of three biological replicates (error bars are SDs from mean values).