TABLE 2.
Selected differentially expressed genes of C. cellulovorans grouped into functional categories in response to cocultivation with R. palustris at mid-exponential growth phase
| Functional category and gene ID | Description | log2 (Coa/C. cellulovorans) |
|---|---|---|
| H2 production (2 genes) | ||
| Clocel 1684 | Pyruvate ferredoxin/flavodoxin oxidoreductase | −1.3 |
| Clocel 4097 | Hydrogenase, Fe only | −2.3 |
| Cellulose degradation (3 genes) | ||
| Clocel 3359 | Cellulase, glycosyl hydrolase family 5 protein | 1.7 |
| Clocel 3111 | Cellulase, glycosyl hydrolase family 5 protein | 2.1 |
| Clocel 2295 | Xylanase, glycosyl hydrolase family 11 protein | 2.5 |
| Fatty acid biosynthesis (3 genes) | ||
| Clocel 4138 | Acetyl-CoA carboxylase, biotin carboxyl carrier protein | 2.7 |
| Clocel 4144 | 3-Oxoacyl-(acyl-carrier-protein) synthase III | 2.6 |
| Clocel 4162 | Beta-ketoacyl-ACP synthase | 1.4 |
| Sulfur metabolism (10 genes) | ||
| Clocel 0525 | Fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein | −5.2 |
| Clocel 0526 | 4Fe-4S ferredoxin | −13.6 |
| Clocel 2532 | O-acetylhomoserine/O-acetylserine sulfhydrylase | −4.8 |
| Clocel 0527 | Phosphoadenosine phosphosulfate reductase (thioredoxin) | −7.3 |
| Clocel 0528 | Sulfate adenylyltransferase, large subunit | −6.0 |
| Clocel 3475 | Cysteine synthase | −5.4 |
| Clocel 0521 | Sulfate ABC transporter, periplasmic sulfate-binding protein | −3.9 |
| Clocel 0524 | Sulfate ABC transporter ATPase | −4.5 |
| Clocel 0523 | Sulfate ABC transporter, inner membrane subunit CysW | −5.5 |
| Clocel 0522 | Sulfate ABC transporter, inner membrane subunit CysT | −5.9 |
| Thiamine metabolism (6 genes) | ||
| Clocel 2812 | Thiamine biosynthesis protein ThiC | −1.9 |
| Clocel 0684 | Thiazole biosynthesis protein ThiH | −2.0 |
| Clocel 0685 | Thiazole biosynthesis family protein | −2.2 |
| Clocel 0683 | Thiamine biosynthesis protein ThiF | −2.6 |
| Clocel 0682 | Thiamine-phosphate pyrophosphorylase | −2.9 |
| Clocel 3433 | Biotin and thiamine synthesis-associated protein | −3.1 |
| Nitrogen metabolism (5 genes) | ||
| Clocel 1684 | Pyruvate ferredoxin/flavodoxin oxidoreductase | −1.3 |
| Clocel 1284 | Glu/Leu/Phe/Val dehydrogenase | −1.9 |
| Clocel 2836 | Nitrogenase iron protein | 2.0 |
| Clocel 2838 | NADH dehydrogenase ubiquinone 24-kDa subunit | 2.0 |
| Clocel 4147 | 2-Nitropropane dioxygenase | 2.1 |
| Molybdate transport (3 genes) | ||
| Clocel 1529 | Molybdenum ABC transporter periplasmic molybdate-binding protein | 2.5 |
| Clocel 1530 | Molybdate ABC transporter inner membrane subunit | 2.0 |
| Clocel 1531 | ABC transporter | 2.1 |
a
Co, cocultures of C. cellulovorans and R. palustris.