FIG 12.

AmVs accumulate and retain the endoplasmic reticulum marker calnexin. Indirect immunofluorescence localization of calnexin using an anti-calnexin antibody (red) in D. andersoni cells that were synchronously infected with A. marginale labeled with anti-Msp2 antibodies (green) or mock infected with medium (uninfected). Calnexin accumulates around AmVs 5 days postinoculation and is retained at 10 days postinoculation. The box in the Merge+DAPI image outlines the area of magnification in the Enlarged column. The uninfected control shows the pattern of calnexin staining in D. andersoni cells. Scale bars represent 10 μm on a 63×/1.20 objective. The white arrowhead in the first column indicates the location of the AmV and the area enlarged in the inset. All images are at the same magnification, with the exception of the insets and last column, which are enlarged 3-fold.