FIG 4.

AmVs accumulate and retain the recycling endosomal compartment marker Rab11. Indirect immunofluorescence localization of Rab11 using anti-Rab11 antibodies (red) in D. andersoni cells that were synchronously infected with A. marginale labeled with anti-Msp2 antibodies (green) or mock infected with medium (uninfected). Recruitment of Rab11 is not apparent 1 h postinoculation. Rab11 accumulates on the majority of AmVs 5 days postinoculation and is retained at 10 days postinoculation. Inhibition of bacterial protein synthesis (Tetracycline Rx) results in loss of Rab11 labeling of the AmVs. The uninfected control shows the pattern of staining of Rab11 in D. andersoni cells. The box in the Merge+DAPI image outlines the area of magnification in the Enlarged column. Scale bars represent 10 μm on a 63×/1.20 objective. The white arrowhead in the first column indicates the location of the AmV and the area enlarged in the inset. All images are at the same magnification, with the exception of the insets and last column, which are enlarged 3-fold.