TABLE 1.
Identification of targeted tick proteins by Western blotting and LC-MS/MS
| Antibody target | Molecular mass (kDa)a | Accession no.b | BLAST homolog descriptionb | PLGS scorec | No. of peptide matchesd | Coverage (%)e |
|---|---|---|---|---|---|---|
| Rab5 | 26 | JAB84144.1 | Putative Ras-related protein Rab5b (Ixodes ricinus) | 265 | 5 | 33 |
| Rab4 | 23 | AAA80151.1 | Rab4 (Dictyostelium discoideum) | 208 | 2 | 12 |
| Rab11 | 24 | JAA58910.1 | Putative Ras-related protein Rab11a (Rhipicephalus pulchellus) | 192 | 5 | 23 |
| Rab7 | 23 | JAB83106.1 | Putative Rab7 member Ras oncoprotein family (Ixodes ricinus) | 707 | 8 | 49 |
| LAMP1 | 120 | XP_007466423.1 | Predicted lysosome-associated membrane glycoprotein 1 (Lipotes vexillifer) | 89 | 4 | 17 |
| Cathepsin L | 37 | JAC35239.1 | Putative cathepsin l-like cysteine proteinase b (Amblyomma triste) | 179 | 4 | 19 |
| Calnexin | 67 | EEH07274.1 | Calnexin (Histoplasma capsulatum) | 123 | 5 | 13 |
| Calreticulin | 50 | AAR29942.1 | Calreticulin (Dermacentor andersoni) | 512 | 6 | 18 |
| 58K Golgi protein | 58 | NP_001192328.1 | Formimidoyltransferase cyclodeaminase/58K Golgi protein (Bos taurus) | 111 | 4 | 6 |
a
Molecular mass of band on SDS-PAGE.
b
Homolog with the highest Protein Lynx Global Server (PLGS) score as identified by the Basic Local Alignment Search Tool (BLAST).
c
PLGS ion scores of ≥18 correspond to a probability ≥95% that the peptide match is not random and indicate identity or extensive homology.
d
Number of peptides identified from each protein.
e
Percentage of coverage of target protein by the identified peptide.