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. 2016 May 5;114(12):1343–1351. doi: 10.1038/bjc.2016.113

Figure 2.

Figure 2

CCR6 expression in colon cancer cell lines. Normal colon epithelial (CRL-1790), Duke's type C (CCL221) and type D (CCL224) cells were stained with FITC-conjugated anti-CCR6 antibodies or isotype antibodies and data was acquired using Guava Flow cytometer and analysed with FlowJo. (A) Surface expression of CCR6. Solid histogram show CCR6 expression and open histogram represents isotype control. (B) Mean fluorescence intensity of CCR6 surface staining with respect to the isotype control in respective cell lines. ***P-value<0.001 between normal colon cells vs colon cancer cells. Copies of CCR6 transcript per million copies of 18S rRNA are shown in C. Values represent the mean±s.e.m. from three independent experiments. ****P-value<0.0001 difference in CCR6 expression in colon cancer cell lines compared with the normal colon cells.