Figure 3.
Effect of CCR6–CCL20 interactions on EMT. Transcript levels of E-cadherin, N-cadherin, β-catenin, Vimentin, α-SMA, SNAIL1, ZEB1 in untreated (open box) and CCL20-treated (solid box) cells were quantified by qRT-PCR and normalised with 18S rRNA. Relative fold change, using ΔΔCt method was calculated with untreated samples as control. Fold change in E-cadherin, N-cadherin, β-catenin, Vimentin, α-SMA, SNAIL1, ZEB1 mRNA transcripts in colon cancer cell lines: CCL221 (A), CCL222 (B), CCL224 (C) and CCL225 (D) are shown on left side. Statistical significance of change in mRNA level of EMT marker in CCL20-treated cells compared with untreated cells are indicated as *P-value<0.05, **P-value<0.001 and ***P-value<0.0001. Western blot analysis was used to confirm mRNA expression results at protein level. GAPDH was used as loading control. Western blot images of EMT markers are shown at right side in A to D.