Skip to main content
. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Eur J Immunol. 2016 May 25;46(7):1783–1796. doi: 10.1002/eji.201546212

Figure 2. I.v. tolerized Lgals1−/− mice have reduced numbers of Tr1 cells and Tregs in the CNS.

Figure 2

MNCs were isolated from the CNS of MOG-i.v. and PBS-i.v. EAE WT (n=7) and Lgals1−/− (n=6) mice on day 25 p.i. (A) MNCs were cultured overnight with MOG35-55 (10 μg/ml), activated with PMA + ionomycin + Golgi plug (PMA/iono/GP), then stained and analyzed by flow cytometry. (B) Representative flow cytometry dot plots showing IL-10 expression in CD4+ T cells. (C) Representative flow cytometry dot plots showing FoxP3 expression on CD4+ T cells. (D) Proportions and absolute numbers of infiltrating Tr1 cells and Tregs were determined for WT and Lgals1−/− MOG-i.v. and PBS-i.v. mice. Pooled data from four experiments are shown. Experiments show mean ± SEM. *, p<0.05, unpaired Student’s t test.