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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Angew Chem Int Ed Engl. 2014 Apr 8;53(19):4867–4871. doi: 10.1002/anie.201402092

Figure 2.

Figure 2

Controlled EGFP expression in 293T cells. A) Chemical structures of 4-azidophenylalanine (AzF), 4-acetylphenylalanine (AcF), and 4-iodophenylalanine (IodoF). B) EGFP with an Amber codon at position 40. C) EGFP expression in the presence of the tRNATyr–AzFRS pair and 1 mm of AzF. D) EGFP expression in the presence of the tRNATyr–AzFRS pair, but without AzF. Scale bars, 200 µm.