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Infection assays with TZM-bl cells. A) Infected with wild-type pSUMA. B) Infected with pSUMA-Tyr59. C) Infected with pSUMA-Trp36Gln127. Scale bars, 200 µm. The doses used for infection are shown as TCID50 values. The wild-type pSUMA was diluted 4-fold and the pSUMA mutants were concentrated 15-fold in order to adjust the infectious dose of each variant to similar levels.
