TABLE 2.
Plasmids and primers used in this study
| Plasmid or primer name | Plasmid description or primer sequencea |
|---|---|
| Plasmids | |
| pAD | pGADT, HA tag, Ampr leu2 |
| pBD | pGBKT, c-Myc epitope tag, Kanr trp1 |
| pAD-comX | pGADT-comX, HA tag, Ampr leu2 |
| pBD-comX | pGBKT-comX, c-Myc epitope tag, Kanr trp1 |
| pBD-W | pGBKT-comW, c-Myc epitope tag, Kanr trp1 |
| pAD-W | pGADT-comW, HA tag, Ampr leu2 |
| pAD-rpoD | pGADT-rpoD, HA tag, Ampr leu2 |
| pAD-T | pGADT-antigen T, HA tag, Ampr leu2 |
| pBD-53 | pGBKT-murine p53, HA tag, Kanr trp1 |
| pBD-lam | pGBKT-lamin, HA tag, Kanr trp1 |
| Primers | |
| JB17 | GTACGGATCCAGGGGAAAATTATGATTAAAGAATTGTAT |
| JB20 | GCATCTCGAGCTAATGGGTACGGATAGTAAACTC |
| JB37 | GTCAGGATCCTTATGTTACAAAAAATTTATGAGCAGATG |
| JB38 | GCATGTCGACTACTAAAATTACCTCAACAAGAAATAAAC |
| JB41 | GTCAGGATCCTTATGTTACAAAAAATTTATGAGCAGATG |
| JB42 | GCATCTCGAGTACTAAAATTACCTCAACAAGAAATAAAC |
| JB45 | GTACGGATCCAGGGGAAAATTATGATTAAAGAATTGTAT |
| JB48 | GTACGGATCCAGGGGAAAATTATGATTAAAGAATTGTAT |
| JB51 | GTACGGATCCCAGCCCTAGAAGAATTGGAACG |
| JB52 | GCATCTCGAGTCAATTTGCTCTTCTGTATAAG |
a
Underlining indicates restriction enzyme recognition sites. HA, hemagglutinin.