FIG 4.
Specific protection in E. faecalis strains with introduced bacI genes that confer immunity against distinct types of Bac41s. The culture supernatants of E. faecalis OG1S harboring a plasmid vector carrying type I Bac41 (pBac41pYI14), type IIa Bac41 (pBac41pYI12), or type IIb Bac41 (pBac41pMGT421) were spotted onto THB soft-agar (0.75%) plates containing the indicator strains. OG1S strains with the introduced immunity gene type I bacI1 (pbacI1pYI14), type I bacI2 (pbacI2pYI14), type IIa bacI2 (pbacI2pYI12), or type IIb bacI2 (pbacI2pMGT421) were used as indicator strains. The plates were incubated at 37°C for 24 h, and the formation of halos was evaluated.