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. 2016 Aug 11;198(17):2379–2390. doi: 10.1128/JB.00348-16

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Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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FIG 5 — Specific protection in E. faecalis strains with introduced bacI genes against chimeric combinations of recombinant BacL₁ and BacA effectors. (A) Recombinant His-tagged BacL₁ and BacA proteins derived from pYI14 (rBacL_1pYI14 and rBacA_pYI14), pYI12 (rBacL_1pYI12 and rBacA_pYI12), or pMGT421 (rBacL_1pMGT421 and rBacA_pMGT421) proteins (400 ng) were separated by SDS-PAGE and stained with Coomassie brilliant blue (CBB). (B) Combinations of recombinant His-tagged BacL₁ and BacA proteins derived from pYI14 (rBacL_1pYI14 and rBacA_pYI14), pYI12 (rBacL_1pYI12 and rBacA_pYI12), or pMGT421 (rBacL_1pMGT421 and rBacA_pMGT421) proteins (25 ng each) were spotted onto THB soft-agar (0.75%) plates containing the indicator strains. OG1S strains with the introduced immunity gene type I bacI1 (pbacI1_pYI14), type I bacI2 (pbacI2_pYI14), type IIa bacI2 (pbacI2_pYI12), or type IIb bacI2 (pbacI2_pMGT421) were used as indicator strains. The plates was incubated at 37°C for 24 h, and the formation of halos was evaluated.