FIG 4.

Inhibition of integrin subunit expression by shRNA knockdown. (A) FPKM (fragments per kilobase of exon per million fragments mapped) values of integrin subunits (circles) expressed in human melanoma cells determined by RNA sequencing, with darker colors indicating larger values, as indicated. Integrins are represented by the circles linked with a line. (B) Integrin subunit mRNA levels of Mel-DSP2 cell lines expressing shRNAs targeting subunits αV (ITGAV), α6 (ITGA6), β1 (ITGB1), β5 (ITGB5), and β8 (ITGB8) quantified by qRT-PCR. Levels of RNA for shRNA knockdown cells (white) are represented as percentages of those of the control Mel-DSP2 cells (black). Statistical analysis was performed using ANOVA (***, P < 0.001). Data shown are the mean results of at least duplicate experiments, with bars indicating the standard error of the mean (SEM).