TABLE 3.
Inhibition testing of recombinant IAV-WSN-nanoLuc strains harboring proteins with specific mutations
| IAV-WSN-nanoLuc HA mutation(s) | EC90 (μM)a |
||
|---|---|---|---|
| GRP-71271 | GRP-103594 | GRP-115249 | |
| None (standard HA) | 0.3 (0.20–0.49) | 0.3 (0.23–0.49) | 0.2 (0.22–0.79) |
| I257T | 0.8 (0.62–1.02) | 1.3 (0.83–2.02) | 1.1 (0.80–1.43) |
| V323I | 0.7 (0.27–0.96) | 1.3 (0.83–2.02) | 0.5 (0.24–1.01) |
| T156A | 1.0 (0.55–2.03) | 3.3 (1.35–3.98) | 2.3 (0.98–1.58) |
| D85N | 1.8 (0.92–3.58) | 3.6 (1.23–10.56) | 4.2 (9.61–10.87) |
| N154S | 1.1 (0.75–1.66) | 1.7 (1.31–2.21) | 2.5 (1.11–5.64) |
| V323I/T156A | >8.0 | >8.0 | >8.0 |
| I257T/V323I/T156A | >8.0 | >8.0 | >8.0 |
| D85N/N154S | >8.0 | >8.0 | >8.0 |
| V323I/N154S | >8.0 | >8.0 | >8.0 |
| D85N/T156A | >8.0 | >8.0 | >8.0 |
| K58Q | >8.0 | >8.0 | >8.0 |
| F110L | >8.0 | >8.0 | >8.0 |
| M59I | >8.0 | >8.0 | >8.0 |
a
Values represent three independent repeats and were calculated through four-parameter variable-slope regression modeling. Numbers in parentheses specify 95% confidence intervals. The highest concentration assessed was 8 μM.