FIG 2.

Predominant Th2 cytokine expression in LP-BM5-infected mice. (A) Production of Th2 cytokines (IL-5, IL-4, IL-10) and Th1 cytokines (IL-2, IL-12, IFN-γ) from splenocytes obtained from uninfected or LP-BM5-infected mice. Infected and uninfected mice were sacrificed at 2 or 5 weeks after infection, spleen cells were cultured, and supernatants were harvested at 72 h for assay for all cytokines. Cytokine assays were performed as described in Materials and Methods. Fold change was calculated for every infected mouse by dividing the concentration of the cytokine (expressed as picograms per milliliter) by the mean value for the corresponding cytokine from uninfected controls. Values represent the means ± SDs for 5 animals. *, P < 0.05 versus uninfected mice at the same time point; **, P < 0.01 versus uninfected mice at the same time point. (B) Expression of the IL-5 gene. The fold change with respect to the value for the uninfected controls was calculated using the 2^−ΔΔCT method with normalization of the raw data to the data for the housekeeping gene GAPDH (glyceraldehyde-3-phosphate dehydrogenase). Data analysis was performed using web-based RT² Profiler PCR array data analysis software (SABiosciences). Values represent the means for 3 animals and were obtained at 2 and 5 weeks postinfection. The error bars represent 95% confidence intervals. *, P < 0.05 versus uninfected mice at the same time point.