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. 2016 Jul 27;90(16):7508–7518. doi: 10.1128/JVI.00012-16

FIG 1.

FIG 1

Fusion activity of the Buenos Aires F protein (BAF) and its effect on attenuation in vitro and in cotton rats. (A) The consensus sequence of the BAF protein was obtained by alignment of 5 clinical isolates from the GenBank database. Disagreements from the consensus sequence are indicated. (B) BAF fusion activity was measured using a dual-split protein (DSP) cell-to-cell fusion assay. A representative graph from three experimental replicates shows means + SEMs of 4 replicate wells. ***, P < 0.0005 using two-way ANOVA and Tukey multiple-comparison test. (C) BEAS-2B cells were infected at an MOI of 0.01 FFU/cell with either kRSV-A2 or kRSV-A2-BAF. Growth curve data are presented as means ± SEMs from 3 experimental replicates titrated in triplicate. *, P < 0.05; **, P < 0.005; ****, P < 0.00005 using two-way ANOVA and Tukey multiple-comparison test. LOD, limit of detection. (D) Groups of 5 cotton rats were infected with either kRSV-A2-line19F or kRSV-A2-BAF. On day 4 postinfection (p.i.), lungs were harvested and viral load was measured by standard plaque assay. Each dot represents one animal, and data represent means ± SDs. **, P < 0.005 by two-tailed Student's t test.