FIG 5.

Luciferase mRNA levels in the nuclear and cytoplasmic fractions in PA-X-transfected cells. 293T cells were transfected with pCAGGS containing the indicated genes together with pCAGGS-Luc for 24 h. The cells were then fractionated into the nuclear and cytoplasmic materials. The total RNA from each fraction was extracted and subjected to Northern blot analysis as described for Fig. 2D. The luciferase mRNA level of the PA-X K134A transfection sample of each fraction was set to 100%. The level of U1 snRNA or 12S rRNA was detected as a nuclear (N) or cytoplasmic (C) marker, respectively, by RT-PCR.