FIG 4.

Multiple regions of pUL93 independently interact with pUL77. 293T cells were cotransfected with plasmids expressing Myc-tagged pUL77 and FLAG-tagged pUL93 or FLAG-tagged pUL93 truncated from either the N terminus or the C terminus (A) and harvested at 48 hpi, and co-IP and IB were performed as indicated (B to E). Empty vector pCMV-Myc or a Myc-UL96 (an unrelated protein) expression plasmid was cotransfected with full-length FLAG-UL93 as a negative control in panel B. The empty vector pCMV-Myc was cotransfected with full-length FLAG-UL93 (FL) and the empty vector p3xFLAG-pCMV-10 was cotransfected with Myc-UL77 as negative controls in panel C.