FIG 8.

Compromised viral proliferation of the HHH viral strain in a single round of infection. A panel of two different reporter viruses, HHC-LGIT (wild type) and HHH-LGIT, was constructed with the TFBS variations engineered in the 3′ LTR. The reporter viruses were pseudotyped with VSV-G envelope, and each virus coexpresses GFP and Tat from the LTR. CEM-CCR5 (A) or Jurkat (B) cells were infected with equivalent TCID₅₀ units of one of the two viral strains, and 6 h later the unbound virus was removed by washing. The cells were activated with TNF-α (100 ng/ml) after 18 h of incubation or left without activation. The mean fluorescence intensity of GFP was monitored using flow cytometry at 48 h (data presented) and 96 h (data not presented) following TNF-α activation. The data are representative of two independent experiments.