FIG 7.

Stress granules do not form in viral inclusions. U2OS cells were infected with EBOV at an MOI of 1.5. At 24 h p.i., cells were treated with 0.5 mM Ars for 30 min, fixed, and analyzed by IF analysis using antibodies against eIF4G (green) and VP35 (red). Representative fields of infected cells are shown. (A) Arrows indicate three examples of SG formation around viral inclusions, shown zoomed 1.4× to the right with separated color channels. (B) Boxed areas are shown zoomed 2.6× below the larger images. Scale bars = 20 μm.