FIG 9.

Excess VP35 disrupts both SGs and viral inclusions. Huh7 cells were transfected with pCAGGS expression plasmids expressing EBOV NP or HA-tagged VP35. Top rows show cells transfected in a 1:1 plasmid ratio at 0.5 μg each per well in a 6-well plate. Cells shown in bottom rows were transfected in a NP-to-VP35 plasmid ratio of 1:3 (0.5 μg of NP DNA and 1.5 μg of VP35 DNA). At 2 days posttransfection, cells were treated with 0.5 mM Ars for 30 min or left untreated and then imaged by IF analysis. Staining was performed using antibodies targeting HA to visualize VP35 (A; red), NP (B; red), and eIF4G (A and B; green). Scale bars = 20 μm.