TABLE 1.

Recovery of mutant viruses using site-directed mutagenesis

nsp12-RdRp region	Engineered substitution (nsp12)		Recovery
	CVB3a	MHV	nsp14-ExoN(+) (WT)	nsp14-ExoN(−)
Fingers	I176V	V553A	No	Not attempted
		V553I	Yes	Yes
	Y268H	Y649H	Yes	No
	Y268W	Y649W	No	Not attempted
Palm	I230F	M611F	Yes	Yes
	F232Y	W613Y	Yes	No
	A239G	A621G	Revertant	Not attempted
	K360R	K794R	Yes	No

^aReference 8.