FIG 3.

Time course of entry of wt and mutant cells. HeLa cells were infected with wt and mutant viruses at an MOI of 10,000 as described in Materials and Methods. At the indicated time points, intracellular viral DNA was collected, and the number of genomes that had entered per cell was measured by RT-PCR. Most of the mutants (gray lines) entered the cells as well as the wt. The wild-type virus bound to the A20 antibody (1:17 ratio) accumulated in cells at about the same rate as that of the wt. Two mutants (D528A and mut22) were significantly defective for cell entry. Data for each time point for each mutant represent the means of results from three separate experiments. Error bars for each time point were omitted for clarity.