FIG 2.

Expression of P3 and/or P3N-PIPO from Cl-RB, Cl-No.30, and BY-CS in PI 226564 plants using a heterologous WClMV vector. (A) Schematic representations of P3, P3N-PIPO, and P3ΔPIPO constructs. The P3 construct was expected to produce P3 accompanied by a small amount of P3N-PIPO as a frameshift product. P3N-PIPO had mutations for expressing P3N-PIPO in the zero frame but no P3. P3ΔPIPO contained a mutation enabling it to produce P3 but not P3N-PIPO. (B and C) P3, P3N-PIPO, and P3ΔPIPO constructs of Cl-RB (B) and Cl-No.30 (C) were expressed by WClMV vectors. The photographs in panels B and C were taken at 5 dpi. (D) P3N-PIPO of BY-CS was expressed by a WClMV vector. The photographs were taken at 14 dpi.