FIG 4.

ALCAM expression on the thoracic spinal cord BBB endothelium is unchanged in HAM/TSP patients. (A) Spinal cord sections of control (n = 3) and HAM/TSP (n = 2) patients were immunostained for factor VIII (left) and ALCAM (right). In the merged image, factor VIII is presented in red, ALCAM in green, and nuclei in blue (4′,6-diamidino-2-phenylindole [DAPI] staining). Observations were performed using an ApoTome.2 microscope. The images are representative of the observed ALCAM and factor VIII immunoreactivities for each individual. Scale bar = 50 μm. (B) Quantification of ALCAM and factor VIII expressions on the BBB. Five distinct cross sections were defined for each vessel presented in panel A. Peak fluorescence signal intensity for each staining is reported. Horizontal line indicates the mean ± SD.