Figure 6.

Effect of Oncamex on xenografts in an in vivo mice model. Treatment of mice implanted with MDA-MB-231 xenografts with Oncamex (25 mg kg⁻¹ per day, treating for 4 consecutive days with a 2-day rest in between) showed to exert a significant growth-inhibitory effect in comparison to untreated animals (A). Mean body weight was registered every second day for 20 days (B). Ki-67 activation was measured by immunohistochemistry in controls (C) and xenografts treated with Oncamex (D). Statistical study reported a statistically significant decrease in expression of the proliferation-linked protein as reported unpaired t-test analysis (E, F). Image analysis with the open-access processing programme ImageJ also showed a statistically significant reduction in the percentage of viable areas in the tissue (E, G). P-values from unpaired t-test: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Where not shown P>0.05 (nonsignificant).