Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1983 Oct;36(10):1140–1144. doi: 10.1136/jcp.36.10.1140

Defective platelet aggregation to the calcium ionophore A23187 in a patient with a lifelong bleeding disorder.

S J Machin, J P Keenan, B A McVerry
PMCID: PMC498490  PMID: 6413552

Abstract

A patient with a lifelong bleeding disorder is presented with a prolonged bleeding time and abnormal aggregation and secretion responses to arachidonic acid, thromboxane A2, PAF-acether and the divalent calcium ionophore A23187. Platelet alpha and dense granule contents and morphology appear normal. The proposed defect is due to an abnormality of a platelet intracellular calcium dependent process.

Full text

PDF
1140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chignard M., Le Couedic J. P., Vargaftig B. B., Benveniste J. Platelet-activating factor (PAF-acether) secretion from platelets: effect of aggregating agents. Br J Haematol. 1980 Nov;46(3):455–464. doi: 10.1111/j.1365-2141.1980.tb05993.x. [DOI] [PubMed] [Google Scholar]
  2. Defreyn G., Machin S. J., Carreras L. O., Dauden M. V., Chamone D. A., Vermylen J. Familial bleeding tendency with partial platelet thromboxane synthetase deficiency: reorientation of cyclic endoperoxide metabolism. Br J Haematol. 1981 Sep;49(1):29–41. doi: 10.1111/j.1365-2141.1981.tb07194.x. [DOI] [PubMed] [Google Scholar]
  3. Detwiler T. C., Charo I. F., Feinman R. D. Evidence that calcium regulates platelet function. Thromb Haemost. 1978 Oct 31;40(2):207–211. [PubMed] [Google Scholar]
  4. Feinstein M. B., Fraser C. Human platelet secretion and aggregation induced by calcium ionophores. Inhibition by PGE1 and dibutyryl cyclic AMP. J Gen Physiol. 1975 Nov;66(5):561–581. doi: 10.1085/jgp.66.5.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hardisty R. M., Machin S. J., Nokes T. J., Rink T. J., Smith S. W. A new congenital defect of platelet secretion: impaired responsiveness of the platelets to cytoplasmic free calcium. Br J Haematol. 1983 Apr;53(4):543–557. doi: 10.1111/j.1365-2141.1983.tb07306.x. [DOI] [PubMed] [Google Scholar]
  6. Haslam R. J., Davidson M. M., Fox J. E., Lynham J. A. Cyclic nucleotides in platelet function. Thromb Haemost. 1978 Oct 31;40(2):232–240. [PubMed] [Google Scholar]
  7. Hattori A., Takahashi H., Takahashi M., Shibata A., Okuma M. A new familial defect of platelet release mechanism (the intracellular Ca++ transport defect?). Nihon Ketsueki Gakkai Zasshi. 1981 Jul;44(4):969–972. [PubMed] [Google Scholar]
  8. Lages B., Malmsten C., Weiss H. J., Samuelsson B. Impaired platelet response to thromboxane-A2 and defective calcium mobilization in a patient with a bleeding disorder. Blood. 1981 Mar;57(3):545–552. [PubMed] [Google Scholar]
  9. Okuma M., Takayama H., Uchino H. Subnormal platelet response to thromboxane A2 in a patient with chronic myeloid leukaemia. Br J Haematol. 1982 Jul;51(3):469–477. doi: 10.1111/j.1365-2141.1982.tb02804.x. [DOI] [PubMed] [Google Scholar]
  10. Russell N. H., Keenan J. P., Bellingham A. J. Thrombocytopathy in preleukaemia: association with a defect of thromboxane A2 activity. Br J Haematol. 1979 Mar;41(3):417–425. doi: 10.1111/j.1365-2141.1979.tb05876.x. [DOI] [PubMed] [Google Scholar]
  11. Samama M., Lecrubier C., Conard J., Hotchen M., Breton-Gorius J., Vargaftig B., Chignard M., Lagarde M., Dechavanne M. Constitutional thrombocytopathy with subnormal response to thromboxane A2. Br J Haematol. 1981 Jun;48(2):293–303. [PubMed] [Google Scholar]
  12. Sultan Y., Jeanneau C., Lamaziere J., Maisonneuve P., Caen J. P. Platelet factor VIII-related antigen: studies in vivo after transfusion in patients with von Willebrand disease. Blood. 1978 Apr;51(4):751–761. [PubMed] [Google Scholar]
  13. Walsh P. N., Biggs R., Gagnatelli G. Platelet antiheparin activity. Assay based on factor-Xa inactivation by heparin and antifactor Xa. Br J Haematol. 1974 Mar;26(3):405–419. doi: 10.1111/j.1365-2141.1974.tb00482.x. [DOI] [PubMed] [Google Scholar]
  14. White G. C., 2nd Calcium-dependent proteins in platelets: response of calcium-activated protease in normal and thrombasthenic platelets to aggregating agents. Biochim Biophys Acta. 1980 Aug 1;631(1):130–138. doi: 10.1016/0304-4165(80)90061-6. [DOI] [PubMed] [Google Scholar]
  15. White G. C., 2nd, Raynor S. T. The effects of trifluoperazine, an inhibitor of calmodulin, on platelet function. Thromb Res. 1980 Apr 1;18(1-2):279–284. doi: 10.1016/0049-3848(80)90194-2. [DOI] [PubMed] [Google Scholar]
  16. Wu K. K., Le Breton G. C., Tai H. H., Chen Y. C. Abnormal platelet response to thromboxane A2. J Clin Invest. 1981 Jun;67(6):1801–1804. doi: 10.1172/JCI110221. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES