. Author manuscript; available in PMC: 2016 Aug 15.

Published in final edited form as: J Dev Biol. 2014 Apr 10;2(2):84–100. doi: 10.3390/jdb2020084

Table 1.

Epicardial lineage tracing following myocardial infarction. Abbreviations: Ad: adenovirus, Msln: mesothelin, SMC: smooth muscle cell, Fibro: fibroblast, EC: endothelial cell, CM: cardiomyocyte, SM-MHC: smooth muscle myosin heavy chain, αSMA: α smooth muscle actin, FN1: fibronectin 1. ColIII: collagen type III, FSP1: fibroblast-specific protein 1, proCol1: procollagen1, cTnI: cardiac TroponinI, sαActin: sarcomeric α Actin, DDR2: discoidin domain receptor2, cTnT: cardiac TroponinT, Cx43: connexin 43; N-Cad: N-Cadherin; Tβ4: Thymosinβ4.

Model	Activation	EPDC Differentiation	Markers Used for Identification	Time-points	Details	Reference
WT1^CreERT2/+; R26R^mTmG	Expansion	SMC Fibro Sporadic EC	SM-MHC, αSMA, SM22α FN1, ColIII, FSP1, ProCol1 PECAM	14d ^#	Tamoxifen: twice weekly for 2-3 weeks, MI one week after final injection	33
Ad:Msln-Cre; R26R^mTmG	n.a.	Fibro	FSP1	3d-4wks	Ultrasound-guided virus delivery	33
WT1^CreERT2/+; R26R^EYFP	Expansion Migration	CM^*	cTnT, sαActin, Cx43, N-Cad, Ca2⁺ transients Functional coupling	14d	Tβ4: Pre- and post-MI Tamoxifen: 5 and 3 days pre- MI	34
Gata5-Cre; R26R^EYFP	Expansion Migration	SMC^$ EC^$ Fibro	αSMA PECAM ProColI	7d	Tβ4: Pre- and post-MI	36
WT1^CreERT2/+; R26R^mTmG	Expansion	SMC Fibro	αSMA DDR2, ProCol1, desmin, FSP1, ColIII	14d	Tamoxifen: twice weekly for 2-3 weeks, MI one week after final injection Tβ4: Post-MI	38
(BAC)WT1^EGFPCre; R26R	Expansion Migration	SMC EC CM^{^} Fibro	αSMA PECAM + location in vessel wall cTnI, SERCA2 via exclusion of other markers	1mo, 3mo (CM)		43
LV-CMVGFP	Migration	CM^*	sαActin, morphology	7d, 21d	Sub-pericardial virus injection	44

other cell types not reported;

comparable results at 1week ,1month and 3 month post-MI;

^{^}

cells lacked adult CM morphology;

displayed no mature markers, remained rounded.