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. 2016 Aug 9;10:2535–2546. doi: 10.2147/DDDT.S110125

Figure 4.

Figure 4

Immunoblotting analysis of RANKL and IL-6 expression in lumbar vertebrae and immunohistochemistry staining for IL-6 expression in the distal femur.

Notes: (A) Representative immunoblotting images of RANKL and IL-6. (B) RANKL expression. (C) IL-6 expression. (D) Immunohistochemistry staining for IL-6 expression, arrows indicate positive expression of IL-6. (scale 100 μm, magnification: 20×) (E) IL-6 expression in the distal femur. WT group, wild type mice; lupus group, lupus mice (B6.MRL-Faslpr/J); GC group, lupus mice with prednisone treatment; Sal group, lupus mice with salvianolate treatment; GC + Sal group, lupus mice with prednisone plus salvianolate treatment. Values are presented as mean ± SD. aP<0.05 vs WT; AP<0.01 vs WT; BP<0.01 vs lupus; GC; CP<0.01 vs GC; dP<0.05 vs Sal; DP<0.01 vs Sal.

Abbreviations: GC, glucocorticoid; IL, interleukin; RANKL, receptor activator of nuclear factor kappa-B ligand; Sal, salvianolate; SD, standard deviation.