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Template-directed primer extension system. (a) Primer extension reaction scheme. A 5′-Cy5-tagged RNA primer anneals to a complementary template. 2-MeImpX analogues form Watson–Crick base pairs on a complementary template and participate in template-directed primer extension. (b) Structure of thiolated uracil and thymine nucleobases in 2-methylimidazole-activated nucleotides.
