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Schematic representation of MiSeq library assembly protocol. (A) Primer extension reaction. (B) Biotinylated-primer/streptavidin bead association. (C) Template removal. (D) Biotinylated primer/streptavidin dissociation. (E) 3′ Adaptor ligation. (F) Primer hybridization. (G) Reverse transcription: First strand cDNA synthesis. (H) PCR enrichment.
