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. 2016 Aug 4;1(12):e87315. doi: 10.1172/jci.insight.87315

Figure 6. Expression levels of neutrophil chemoattractants are decreased in donor macrophage populations in TLR9-deficient heart grafts.

Figure 6

Expression levels of CXCL2 and CXCL5 in sorted donor-derived (CD45.2+CD45.1–CD11b+CD64+) CCR2+ and CCR2– macrophages examined 2 hours after transplantation of B6 CD45.2+ WT or B6 CD45.2+ MyD88–deficient hearts into congenic B6 CD45.1+ recipients. Results were normalized to 18s RNA and compared with the CCR2– macrophage population in each experimental group. *P < 0.05; **P < 0.01 (2-way ANOVA). Filled circles, WT CCR2- macrophages; filled squares, WT CCR2+ macrophages and monocyte-derived macrophages; filled triangles, TLR9-deficient CCR2– macrophages; open circles, TLR9-deficient CCR2+ macrophages and monocyte-derived macrophages. Graph represents at least 4 separate experiments per group where cells from 2 hearts were pooled for each experiment. Horizontal bars denote means, and error bars denote ±SEM.