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Comparisons of staining for serotonin in horizontal sections in brainstem tissue that was fixed with buffered 4% paraformaldehyde with added 2.5% acrolein, versus that fixed with only buffered 4% paraformaldehyde. The inclusion of acrolein in the fixative impairs recognition of the tissue serotonin by the antibody. For the color version of this figure go to http://www.currentprotocols.com.
