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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Curr Protoc Neurosci. 2016 Jul 1;76:2.12.1–2.12.37. doi: 10.1002/cpns.1

Table 2.12.1.

Comparison of ICC Methods

Method Concentration/ABC NiDAB Comments
ABC–NiDAB 1 Most sensitive; strongest staining at lowest concentration
Direct tagged secondary antibody 20- to 100-fold higher concentration of primary antibody to get maximal staining Least sensitive of the fluorescence methods
ABC–streptavidin fluorescence 10- to 30-fold higher concentration of primary needed to obtain maximal staining Approximately 3 times more sensitive than the direct tagged secondary fluorescence
Biotinylated TSA-amplified streptavidin fluorescence 2-fold higher concentration of primary antibody yields equal sensitivity to the NiDAB method Most sensitive of the fluorescence methods