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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Curr Protoc Neurosci. 2016 Jul 1;76:2.12.1–2.12.37. doi: 10.1002/cpns.1
Summary of MW Program 5:
Tyramide Signal Amplification with BT followed by rinsing Microwave Settings
Time Wattage
1. BT incubation 7 mina 150
2. PBS rinse 4 × 1 minb 150
a
3 min on, 1 min off, 3 min on
b
Unit will pause between each rinse to allow moving the nets between cups