Summary of MW Program 4
Rinsing, secondary antibody and ABC	Microwave Settings
	Time	Wattage
1. PBS rinse	3 × 1 mina	150
2. Secondary antibody	19 minb	150
3. PBS rinse	3 × 1 mina	150
4. A/B reagent	19 minb	150

^a

Unit will pause between each rinse to allow moving the nets between cups

^b

5 min on, 2 min off, 5 min on, 2 min off, 5 min on