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(A) In vitro expression of the putative glutamate transporters (bb0401 and gltP). Gene expression was normalized to enoS. (B) gltP expression during nymph feeding (C). Rate of growth from 150 to 1050 mOsm in microaerobic condition. (D) Immunoblots of B. burgdorferi B31-5A18 and B31-5A18gltP grown at 300, 450 and 650 mOsm to mid-log phase and cell lysates (40μg of protein/lane) probed with OspC-specific antisera.
