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. 2016 Aug 15;12(8):e1005826. doi: 10.1371/journal.ppat.1005826

Fig 7. The activation of ILYS-3 does not require MPK-1 activity in the gut.

Fig 7

(A) Images of single and double transgenic animals carrying the ilys-3p::GFP reporter without or with the transgene mtl-2p::MPK-1 in the WT (N2) and in the mpk-1(ku1) backgrounds. The construct mtl-2p::MPK-1 drives MPK-1 expression in the intestine (int). In the mpk-1 mutants, ilys-3 expression was blocked. This phenotype was not rescued when MPK-1 is restored in the intestine. (B) Quantification of fluorescence intensity in the intestinal cell int2. Asterisks indicate the results of a Mann–Whitney Unpaired test statistical comparisons of the fluorescence intensity for mpk-1(ku1); ilys-3p::GFP; mtl-2p::MPK-1vs ilys-3p::GFP; mtl-2p::MPK-1(*** p = 0.0005) and mpk-1(ku1); ilys-3p::GFP vs ilys-3p::GFP (*** p = 0.0002). Mean values for mpk-1 mutants with the double transgene were not significantly different (NS) from their sibling controls harbouring the ilys-3p::GFP reporter only (p = 0.0934). N = 10-15/group.