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. 2016 Aug 15;11(8):e0161247. doi: 10.1371/journal.pone.0161247

Fig 5. The inhibitory effect of adiponectin on hBD2 expression signaling molecules on repeated UVB exposed NHEK.

Fig 5

(a) The time dependent phosphorylation of MAPKs induced by repeated UVB exposure. Adiponectin attenuated the phosphorylation of (b) JNK/SAPK, ERK and p38 MAPK phosphorylation (the relative expression levels were quantified and presented in graphical form) and (c) c-Fos and c-Jun protein expression. The UVB induced upregulation of hBD2 was suppressed through (d) the AP-1 components c-Fos and c-Jun. NHEK were treated with both SP600125 and SB203580 inhibitors before UVB exposure. Protein expression levels were analyzed by Western blot. And the relative (e) mRNA expression of c-Fos, c-Jun and hBD2 are represented in graphical form (fold change compared with NC cells). Data are presented as the mean ± SEM of three independent experiments (n = 3). *, p<0.05, vs. NC. #, p<0.05, vs. UVB treated group.