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. 2016 Aug 15;14(8):e1002534. doi: 10.1371/journal.pbio.1002534

Fig 7. Sec4p overrides the inhibition of cortical actin polymerization to induce compensatory endocytosis.

Fig 7

Our results supported the model in which, after the completion of polarized exocytosis, activated GTP-Sec4p deposited on the plasma membrane (PM) physically interacts with Las17p. GTP-bound Sec4p then overrides Sla1p inhibition of Las17p to promote both Arp2/3-dependent actin polymerization and the ensuing steps of endocytic membrane internalization. In the immediate vicinity around polarized sites where exocytic vesicles fuse with the PM, Sec4p directly integrates membrane transport to and from the cell surface by inducing compensatory endocytosis. In this manner, GTP-bound Sec4p promotes cortical actin polymerization after coat and adaptor proteins have initiated the formation of nascent endocytic sites.