Table 1. E. coli expression systems characterized in this study.
| System | UptakeMechanism | Inducer | Inducer concentrations* | E. coli strain (plasmid) | Cultivation temperature | References |
|---|---|---|---|---|---|---|
| PT7lac/LacI | active(lacY+) | IPTG | 0, 0.05, 0.1 mM | BL21(DE3) (pRhotHi-2-EYFP) | 37°C | [47] |
| PT7lac/LacI | passive (lacY-) | IPTG | 0, 0.05, 0.1 mM | Tuner(DE3) (pRhotHi-2-LacI-EYFP) | 37°C | [14] |
| PT7lac/LacI | active (galP+ lacY+) | galactose | 0, 0.4, 1 mM | BL21(DE3)** (pRhotHi-2-LacI-EYFP) | 37°C | [14] |
| PBAD/AraC | active (araEFGH+) | arabinose | 0, 1, 2.5 mM | Tuner(DE3)*** (pAra-GFPmut3) | 37°C | [48] |
| PM1-17/XylS | passive | m-toluic acid | 0, 0.05, 0.1 mM | Tuner(DE3) (pM-117-R45T-GFPmut3) | 30°C | [6,28] & this study |
| PM1-17/XylS | passive | salicylic acid | 0, 0.5, 1.5 mM | Tuner(DE3) (pM-117-R45T-GFPmut3) | 30°C | [6,28] & this study |
* w/o inducer, intermediate inducer concentrations, high inducer concentrations
** galK- strain: inability to metabolize galactose, enables sufficient galactose accumulation for induction
*** araBAD+ strain: metabolizes arabinose, increased inducer concentrations are essential