Table 4. Comparison of morphologically-identified species with sequence clusters (mOTU) for CO1 and 16S at 1–4% thresholds, and corresponding top hits on BLAST above 96% identity.
| Identity Based on Morphology | CO1 | 16S | |||
|---|---|---|---|---|---|
| Family | Species | Congruence with sequence clusters | Top Hits on BLAST (> 96% identity) | Congruence with sequence clusters | Top Hits on BLAST (> 96% identity) |
| Cyrenidae | Batissa violacea | Yes (1–4%) | Batissa violacea DQ837727 (97) | Yes (1–4%) | — |
| Corbicula cf. fluminea | Yes (1–4%) | Corbicula sp. GU781082 (100) | No (1–4%) | Corbicula colorata JX399588 (99) | |
| Corbicula sp. | Yes (1–4%) | Corbicula moltkiana AY275660.1 (100) | No (1–4%) | Corbicula largillierti AB522658 (99) | |
| Unionidae | Hyriopsis bialata | Yes (1–4%) | — | N/A | N/A |
| Hyriopsis desowitzi | N/A | N/A | Yes (1–4%) | — | |
| Parreysia burmana | Yes (1–4%) | — | Yes (1–3%), no (4%) | Parreysia olivacea KP795044 (96) | |
| Parreysia sp. | Yes (1–4%) | Parreysia tavoyensis JN243901 (99) | Yes (1–3%), no (4%) | Parreysia tavoyensis KP795043 (99) | |
| Pilsbryoconcha exilis | Yes (1–4%) | — (matched to same species KP795024 at 90%) | N/A | N/A | |
| Scabies crispata | N/A | N/A | Yes (1–4%) | Scabies crispata KP795048 (97) | |
| Sinanodonta woodiana | Yes (1–4%) | Sinanodonta woodiana KJ434487 (99) | N/A | N/A | |
| Ampullariidae | Marisa cornuarietis | Yes (1–4%) | Marisa cornuarietis KM100140 (98) | N/A | N/A |
| Pomacea canaliculata | Yes (1–4%) | Pomacea canaliculata KJ739609 (99) | N/A | N/A | |
| Pomacea diffusa | Yes (1–4%) | Pomacea diffusa EF515065 (100) | N/A | N/A | |
| Bithyniidae | Bithynia sp. | Yes (1–4%) | — | Yes (1–4%) | — |
| Lymnaeidae | Radix rubiginosa | Yes (1–4%) | — (matched to same species GU451737 at 95%) | Yes (1–4%) | Radix rubiginosa U82076.2 (100) |
| Nassariidae | Anentome bockii | Yes (1–4%) | — | N/A | N/A |
| Anentome helena | Yes (1–4%) | — | N/A | N/A | |
| Neritidae | Clithon corona | Yes (1–3%), no (4%) | — (matched to same species EU732362 at 93%) | N/A | N/A |
| Clithon diadema | Yes (1–4%) | — | Yes (1–3%), no (4%) | Clithon spinosus AY771224 (96) | |
| Clithon lentiginosum | Yes (1–3%), no (4%) | — | Yes (1–4%) | — | |
| Clithon mertoniana | Yes (1–4%) | — | Yes (1–3%), no (4%) | — | |
| Neripteron auriculata | Yes (1–4%) | — | Yes (1–4%) | — | |
| Neritina iris | Yes (1–4%) | — | N/A | N/A | |
| Neritina juttingae | Yes (1–4%) | — | Yes (1–4%) | — | |
| Neritina violacea | Yes (1–4%) | Neritina violacea JX411691 (99) | N/A | N/A | |
| Neritodryas cornea | Yes (1–4%) | — | N/A | N/A | |
| Septaria porcellana | N/A | N/A | Yes (1–4%) | Septaria porcellana AY771228 (98) | |
| Vittina coromandeliana | Yes (1–4%) | Neritina turrita JX411698 (99) | N/A | N/A | |
| Vittina turrita | Yes (1–4%) | — | N/A | N/A | |
| Vittina waigiensis | Yes (1–4%) | Neritina turrita AY820497 (100) | Yes (1–4%) | — | |
| Pachychilidae | Brotia armata | N/A | N/A | No (1–4%) | Brotia armata AY330810 (100) |
| Brotia binodosa | N/A | N/A | No (1–4%) | Brotia sp. FJ377079 (100) | |
| Brotia herculea | Yes (1–4%) | — | N/A | N/A | |
| Sulcospira tonkiniana | Yes (1–4%) | Sulcospira tonkiniana FJ377297 (99) | N/A | N/A | |
| Tylomelania towutica | Yes (1–4%) | Tylomelania towutica KJ850891 (99) | N/A | N/A | |
| Tylomelania sp. | Yes (1–4%) | Tylomelania sp. KJ850861 (99) | Yes (1–4%) | Tylomelania centaurus AY311829 (99) | |
| Physidae | Physa sp. | Yes (1–4%) | — | N/A | N/A |
| Planorbidae | Gyraulus convexiusculus | Yes (1–4%) | — | N/A | N/A |
| Indoplanorbis exustus | Yes (1–4%) | Indoplanorbis exustus GU451743 (99) | N/A | N/A | |
| Semisulcospiridae | Semisulcospira sp. | Yes (1–4%) | — | N/A | N/A |
| Thiaridae | Melanoides tuberculata | Yes (1–4%) | Melanoides tuberculata KP284138 (100) | N/A | N/A |
| Stenomelania offachiensis | Yes (1–4%) | — | Yes (1–4%) | Stenomelania sp. AY010518 (97) | |
| Stenomelania plicaria | Yes (1–4%) | — | N/A | N/A | |
| Stenomelania cf. plicaria | Yes (1–4%) | — | N/A | N/A | |
| Stenomelania sp. | Yes (1–4%) | — | Yes (1–4%) | — | |
| Thiara cancellata | Yes (1–4%) | — | Yes (1–4%) | — | |
| Viviparidae | Filopaludina cambodjensis | Yes (1–4%) | — | N/A | N/A |
| Filopaludina peninsularis | Yes (1–4%) | — | Yes (1–4%) | — | |
| Sinotaia guangdungensis | Yes (1–4%) | *Bellamya heudei guangdungensis AY296827 (99) | N/A | N/A | |
| Taia pseudoshanensis | N/A | — | Yes (1–4%) | — | |
“N/A” indicates barcodes were not obtained; “—”indicate that top hits on BLAST were <96%.
*Bellamya heudei guangdungensis is a synonym of Sinotaia guangdugensis [73].