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Plaque size comparison. Plaque morphology of wtATV as compared to ATVΔ40L, ATVΔ54R ATVΔ57R and ATVΔ25R. ATV ORFs 40L, 54R and 57R are non-essential gene in cells in culture (Jancovich and Jacobs, 2011). Each non-essentail ATV gene (i.e. ATVΔ40L, ATVΔ54R and ATVΔ57R) show a slight reduction in plaque size as compared to wtATV. In contrast, deletion of the ATV 25R ORF shows a much reduced plaque size compared to either the wtATV or any other recombinant virus.
