Table 2.
Primers used in this study.
| Name1 | Sequence (5′ – 3′) |
|---|---|
| AF-CMV for | GGTATAGGCGGAAGCGCCATGATGTACGGGCCAGATATACG |
| AR-NeoR rev | GTCTTCTTCGCTAATCAGTTTCTGTTCTCAGAAGAACTCGTCAAGAAGG |
| 11R seq for | TGTTATTCGTGGAGGCTG |
| 11R seq rev | ACAGAGACATTCCAGTCG |
| 11R LA for 1k | GTGATACGCACTCTCACTTGC |
| 11R LA for 500 | CGTCATGACCTGCACCG |
| 11R AF LA rev | GGCGCTTCCGCCTATACCTGCATTTTAAATGGACCCC |
| 11R RA rev 1k | GGCTGCAGACCCGGAACAG |
| 11R RA rev 500 | AACCCAATCGGACTTTGG |
| 11R AR RA for | GAACAGAAACTGATTAGCGAAGAAGACGGGATATAATCCAGAGATGAAC |
| 25R seq for | TCTCTCTGAAAAGACATTGC |
| 25R seq rev | GTTACACCTCATTCTCACG |
| 25R LA for 1k | AGTGTTTAGGGACTGGAAAGACG |
| 25R LA for 500 | GCAAGTGTAGGGTTCTCC |
| 25R AF LA rev | CATGGCGCTTCCGCCTATACCCTCGAGTGACTTGTTTTTC |
| 25R RA rev 1k | TCTCTCGGACTATGTGTCG |
| 25R RA rev 500 | CGAGTATAAATGTTGTACCAC |
| 25R AR RA for | GAACAGAAACTGATTAGCGAAGAAGACATAGAGTTTTAAACCTTTTCC |
| 40L seq for | GATGCACTGCCAATACAG |
| 40L seq rev | AAAACACTATTGCAAACACC |
| 40L LA for 1k | ACAAAATTGGATGGATGCATG |
| 40L LA for 500 | TAAAGCTCTCTGAGCACG |
| 40L AF LA rev | GGCGCTTCCGCCTATACCGGCTTATTGTGTAAAGCTGG |
| 40L RA rev 1k | AGTGCGGTCCAGTTTGCG |
| 40L RA rev 500 | ACGTGATACTTCCAGTCG |
| 40L AR RA for | GAACAGAAACTGATTAGCGAAGAAGACATAGAGATTAAGGACTTGTAGC |
| 53R seq for | TTTCGGGACCATTCACAG |
| 53R seq rev | GGTTGGAAACTAGCTAGC |
| 53R LA for 1k | ATGATCTTGGCGTAAGCC |
| 53R LA for 500 | CGATCGGCAACAGTCTAAG |
| 53R AF LA rev | GGCGCTTCCGCCTATACCTGTTGCGAGTTTAGTTTTGG |
| 53R RA rev 1k | TCCAGGATGGTGTACCTG |
| 53R RA rev 500 | TTACGGCGGCAACTGTCC |
| 53R AR RA for | GAACAGAAACTGATTAGCGAAGAAGACGCTGTAAAATAGTTTAGAGAC |
| 54R seq for | GATTCTATCACAACATTTTTAG |
| 54R seq rev | GAAAATATTTTATCACAAGATAC |
| 54R LA for 1k | GTTTTTTGCAACCGCACC |
| 54R LA for 500 | AAAACTGTGACGTTTGTGC |
| 54R AF LA rev | CATGGCGCTTCCGCCTATACC-TTGCTGAACCTGACGCAC |
| 54R RA rev 1k | GTGTCCTCTTTGGTCCTG |
| 54R RA rev 500 | CTAGAACAAGAATGAGGTG |
| 54R AR RA for | GAACAGAAACTGATTAGCGAAGAAGACGGCTTCTTAAACTGTTTCC |
1
GFP = green fluorescent protein; NeoR = neomycin phosphotransferase resistance gene; CMV = cytomegalovirus promoter; AF = adapter forward; AR = adapter reverse; for = forward; rev = reverse; seq = sequencing primer, ~50 bp from start/end of gene; 1k = ~1,000 bp from start/end of gene; 500 = ~500 bp from start/end of gene.