FIGURE 1:

The novel protein Rng10 is important for cell integrity. (A, B) rng10Δ leads to cell lysis. (A) DIC images of cells grown at 36°C for 4 h. Arrows mark examples of lysed cells, which are evident by the shrunken cytoplasm. (B) Quantification of cell lysis in wt and rng10Δ cells at 25°C and 4 h at 36°C. Data are mean ± 1 SD in this and other figures except where noted. The number n is the total from three experiments. *p < 0.0001 compared with wt in t test. (C) Time-lapse images of cell-lysis scenarios (left) and percentage (right) during cell separation at 25°C. No (top), one (middle), or both (bottom) daughter cells lysed. (D) Quantification of cell length of wt and rng10Δ cells with forming or complete septa at 25°C. *p < 0.01 compared with wt. (E) Micrographs (left) and quantifications (right) of polarized growth in wt and rng10Δ cells stained with Calcofluor. Arrows mark growing ends, and arrowheads mark nongrowing ends that lack Calcofluor staining. (F) Septation index of wt and rng10Δ cells at 25°C or 4 h at 36°C. Bars, 5 μm.